A reproducible methodology for absolute viral quantification and viability determination in mechanical inoculations of wheat streak mosaic virus
نویسندگان
چکیده
Abstract Wheat streak mosaic virus (WSMV) is a common wheat causing economic losses to production in the Great Plains of North America. Reproducible inoculation WSMV by mechanical methods essential evaluate resistance breeding lines and relies on successful infectivity particles. We used reverse transcription-quantitative PCR (RT-qPCR) for absolute quantification viral genome copy numbers both inoculum infected leaves. A time-course study was designed determine viability over time as well number related phenotypic rating scale. In phosphate buffer, stable with average 1.86 × 10 6 ± 4.85 5 . Plants inoculated this buffer using finger rub contained leaves ranging between 2.66 4 4.69 at 21 28 days post-inoculation. Viral were statistically similar immediately those later points. There weak linear relationship score model explaining 40% variability (R 2 = 0.40) indicating difficulty disease assessment based solely symptoms. This work describes an accurate methodology quantify concentration plants, emphasizes demand measurement load validate subjective unbiased assessment.
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ژورنال
عنوان ژورنال: Tropical Plant Pathology
سال: 2022
ISSN: ['1983-2052', '1982-5676']
DOI: https://doi.org/10.1007/s40858-022-00507-y